Difference between revisions of "Part:BBa K4765022"
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| − | We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 BBa_K4765119] and [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120] to test the effiency of twister ribozyme and stem-loop. | + | We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 BBa_K4765119]and [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120]to test the effiency of twister ribozyme and stem-loop. |
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Latest revision as of 12:22, 12 October 2023
mScarlet
Introduction
mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)[1].
Usage and Biology
We constructed mScarlet into the composite BBa_K4765119and BBa_K4765120to test the effiency of twister ribozyme and stem-loop.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
=Reference
- ↑ Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.