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===Introduction===
 
===Introduction===
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mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)<ref>Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.</ref>
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mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)<ref>Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. ''Nature methods, 14''(1), 53–56. https://doi.org/10.1038/nmeth.4074.</ref>.
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===Usage and Biology===
 
===Usage and Biology===
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We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 ribozyme measurement module: twister ribozyme (constitutive expression)] and [https://parts.igem.org/Part:BBa_K4765120 ribozyme measurement module: twister ribozyme (leakage expression)] to test the effiency of twister ribozyme and stem-loop.
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We constructed mScarlet into the composite [https://parts.igem.org/Part:BBa_K4765119 BBa_K4765119]and [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120]to test the effiency of twister ribozyme and stem-loop.
  
 
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Latest revision as of 12:22, 12 October 2023


mScarlet

contributed by Fudan iGEM 2023

Introduction

mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns)[1].

Usage and Biology

We constructed mScarlet into the composite BBa_K4765119and BBa_K4765120to test the effiency of twister ribozyme and stem-loop.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


=Reference

  1. ↑ Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., & Gadella, T. W., Jr (2017). mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature methods, 14(1), 53–56. https://doi.org/10.1038/nmeth.4074.