Difference between revisions of "Part:BBa K4790073"

 
 
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<partinfo>BBa_K4790073 short</partinfo>
 
<partinfo>BBa_K4790073 short</partinfo>
  
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The DNA fragment has been site-directed mutated through PCR. The 6th amino acid in expression product of node 59 was mutated from tyrosine to alanine.
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Mutate Y6 of ASR1 (node 59) to A6 by site-directed mutagenesis.
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<!-- Add more about the biology of this part here

Latest revision as of 12:19, 12 October 2023


node59-Y6A

Mutate Y6 of ASR1 (node 59) to A6 by site-directed mutagenesis.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]