Difference between revisions of "Part:BBa K283029"
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<partinfo>BBa_K283029 short</partinfo>
 
<partinfo>BBa_K283029 short</partinfo>
  
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This part includes the coding region for the LuxI protein. It does not contain any rbs or terminator.
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This part used to construct a device which can generate repressor of pTet regulator consitutively. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. We inserted cheZ after pTet in order to control the gene expression by adding inducer.
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<!-- Add more about the biology of this part here
 
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Revision as of 20:43, 20 October 2009

constitutive promoter-rbs-tetR(LVA)

This part used to construct a device which can generate repressor of pTet regulator consitutively. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. We inserted cheZ after pTet in order to control the gene expression by adding inducer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]