Difference between revisions of "Team:USTC/Template/promoter protocols"

(New page: Promoter measurements: BBa_k176011, BBa_k176012, BBa_k176013, BBa_k176014, BBa_k176015, BBa_k176016 1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 . 2. I...)
 
 
Line 1: Line 1:
−
Promoter measurements: BBa_k176011, BBa_k176012, BBa_k176013, BBa_k176014, BBa_k176015, BBa_k176016
 
−
 
 
1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .
 
1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .
  

Latest revision as of 20:34, 20 October 2009

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.