Difference between revisions of "Part:BBa K243010"

Revision as of 19:24, 20 October 2009

His-FluA-Split Linker-Fok_i

These part units some of our parts. The combination of a Histidin-Tag, a Anticalin-Tag and the linked protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluoresceinA tag allows the measurement by [quenching] and the coupling to an flurescein linked oligo.

Purification

We purified this part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot.

Western Blot: His-FluA-SplitLi-Fok_i in pEx;
lanes: NEB prestained marker broad range,
pool of elution fractions 2-5, empty lane, 3 positive controls

Sequence and Features