Difference between revisions of "Part:BBa K4619003"
 
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<partinfo>BBa_K4619003 short</partinfo>
 
<partinfo>BBa_K4619003 short</partinfo>
  
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This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from *V. cholerae* to *E.coli* and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade.
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This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from <i>V. cholerae</i> to <i>E.coli*</i>and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade.
 
Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.
 
Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K461903 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4619003 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 11:19, 12 October 2023


TcpP-LBD

This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from V. cholerae to E.coli*and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade. Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]