Difference between revisions of "Part:BBa K4757062:Design"

Latest revision as of 11:13, 12 October 2023

TPA sensing XylS-K38R-L224Q/Pm expressing mKate2

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 212
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 934
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Ps1/Ps2 tf only works with co-induction of MBA andTPA

Source

XylS/Pm is natively found in P.putida and was first discovered on the TOL Plasmid.

References

Gallegos, M. T., Marqués, S., & Ramos, J. L. (1996). Expression of the tol plasmid xylS gene in pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and Alpha 54-dependent tandem promoters according to the compound used for Growth. Journal of Bacteriology, 178(8), 2356-2361. https://doi.org/10.1128/jb.178.8.2356-2361.1996

Gawin, A., Valla, S., & Brautaset, T. (2017). The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering. Microbial biotechnology, 10(4), 702-718. <a href="https://doi.org/10.1111/1751-7915.12701">https://doi.org/10.1111/1751-7915.12701</a>

Li, J., Nina, M. R. H., Zhang, X., & Bai, Y. (2022). Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution. ACS synthetic biology, 11(3), 1106-1113. <a href="https://doi.org/10.1021/acssynbio.1c00275">https://doi.org/10.1021/acssynbio.1c00275</a>

@@ Line 16: / Line 16: @@
 ===References===
+<p>Gallegos, M. T., Marqués, S., & Ramos, J. L. (1996). Expression of the tol plasmid xylS gene in pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and Alpha 54-dependent tandem promoters according to the compound used for Growth. <i>Journal of Bacteriology</i>, <i>178</i>(8), 2356-2361. https://doi.org/10.1128/jb.178.8.2356-2361.1996</p>
+<p>Gawin, A., Valla, S., & Brautaset, T. (2017). The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering. <i>Microbial biotechnology</i>, <i>10</i>(4), 702-718. <a href="https://doi.org/10.1111/1751-7915.12701">https://doi.org/10.1111/1751-7915.12701</a></p>
+<p>Li, J., Nina, M. R. H., Zhang, X., & Bai, Y. (2022). Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution. <i>ACS synthetic biology</i>, <i>11</i>(3), 1106-1113. <a href="https://doi.org/10.1021/acssynbio.1c00275">https://doi.org/10.1021/acssynbio.1c00275</a></p>