Difference between revisions of "Part:BBa K4694002"

Revision as of 11:11, 12 October 2023

His-QQ-7

Usage and Biology

The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as Candida albicans and Staphylococcus epidermidis [1]. The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.

This sequence is taken from GenBank JX870909. Forbidden restriction sites were removed, prefix and suffix sequences compatible with TypeIIS cloning were added, and the sequence was synthesised by IDT.

For expression in L. plantarum, this CDS was inserted into plasmid pX1845 via TypeIIs cloning. The plasmid has an E. coli origin of replication (pUC18) and antibiotic resistance gene (𝛽-lactamase) to allow for cloning in E. coli DH5𝛼, and an origin of replication and antibiotic resistance gene to allow for propagation in L. plantarum. Three constitutive promoters were tested: synthetic promoter P_48 [2], natural promoter from L. plantarum WCFS1 P_ldhL1 (GenBank NC_004567) and natural promoter from L. lactis P_32 [3]. The latter two promoters had integrated RBS sequences but P_48 was combined with the synthetic RBS SDOPT8 [4]. All constructs contained a terminator from L. lactis MG1363 pepN, called Lacto_term (GenBank AM406671).

Characterisation

References

[1] Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of Candida albicans and Staphylococcus epidermidis. PLoS One, 14, e0211366. [2] Rud, I., Jensen, P. R., Naterstad, K. & Axelsson, L. 2006. A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum. Microbiology, 152, 1011-1019. [3]Liu, W. B., Lin, Z. W., Zhou, Y. & Ye, B. C. 2021. Overexpression of Capsular Polysaccharide Biosynthesis Protein in Lactobacillus plantarum P1 to Enhance Capsular Polysaccharide Production for Di-n-butyl Phthalate Adsorption. J Microbiol Biotechnol, 31, 1545-1551. [4] Tauer, C., Heinl, S., Egger, E., Heiss, S. & Grabherr, R. 2014. Tuning constitutive recombinant gene expression in Lactobacillus plantarum. <i>Microb Cell Fact, 13, 150.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 This sequence is taken from GenBank JX870909. Forbidden restriction sites were removed, prefix and suffix sequences compatible with TypeIIS cloning were added, and the sequence was synthesised by IDT.
-For expression in <i>L. plantarum</i>, this CDS was inserted into plasmid pX1845 via TypeIIs cloning. The plasmid has an <i>E. coli</i> origin of replication (pUC18) and antibiotic resistance gene (𝛽-lactamase) to allow for cloning in <i>E. coli</i> DH5𝛼, and an origin of replication and antibiotic resistance gene to allow for propagation in <i>L. plantarum</i>. Three constitutive promoters were tested: synthetic promoter P_48 (Rud et al., 2006), natural promoter from <i>L. plantarum</i> WCFS1 P_ldhL1 (NC_004567) and natural promoter from <i>L. lactis</i> P_32 (Liu et al., 2021). The latter two promoters had integrated RBS sequences but P_48 was combined with the synthetic RBS SDOPT8 (Tauer et al., 2014). All constructs contained a terminator from<i> L. lactis</i> MG1363 pepN, called Lacto_term (AM406671).
+For expression in <i>L. plantarum</i>, this CDS was inserted into plasmid pX1845 via TypeIIs cloning. The plasmid has an <i>E. coli</i> origin of replication (pUC18) and antibiotic resistance gene (𝛽-lactamase) to allow for cloning in <i>E. coli</i> DH5𝛼, and an origin of replication and antibiotic resistance gene to allow for propagation in <i>L. plantarum</i>. Three constitutive promoters were tested: synthetic promoter P_48 [2], natural promoter from <i>L. plantarum</i> WCFS1 P_ldhL1 (GenBank NC_004567) and natural promoter from <i>L. lactis</i> P_32 [3]. The latter two promoters had integrated RBS sequences but P_48 was combined with the synthetic RBS SDOPT8 [4]. All constructs contained a terminator from<i> L. lactis</i> MG1363 pepN, called Lacto_term (GenBank AM406671).
 ===Characterisation===
@@ Line 14: / Line 14: @@
 ===References===
 [1] Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of <i>Candida albicans</i> and <i>Staphylococcus epidermidis</i>. <i>PLoS One</i>, 14, e0211366.
+[2] Rud, I., Jensen, P. R., Naterstad, K. & Axelsson, L. 2006. A synthetic promoter library for constitutive gene expression in <i>Lactobacillus plantarum</i>. <i>Microbiology</i>, 152, 1011-1019.
+[3]Liu, W. B., Lin, Z. W., Zhou, Y. & Ye, B. C. 2021. Overexpression of Capsular Polysaccharide Biosynthesis Protein in <i>Lactobacillus plantarum</i> P1 to Enhance Capsular Polysaccharide Production for Di-n-butyl Phthalate Adsorption. <i>J Microbiol Biotechnol</i>, 31, 1545-1551.
+[4] Tauer, C., Heinl, S., Egger, E., Heiss, S. & Grabherr, R. 2014. Tuning constitutive recombinant gene expression in <i>Lactobacillus plantarum. <i>Microb Cell Fact</i>, 13, 150.
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