Difference between revisions of "Part:BBa K4790073"

 
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<partinfo>BBa_K4790073 short</partinfo>
 
<partinfo>BBa_K4790073 short</partinfo>
  
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The DNA fragment has been site-directed mutated through PCR. The 6th amino acid in expression product of node 59 was mutated from tyrosine to alanine.
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ASR1(node59) is constructed on pET-22b(+) vector by Gibson Assembly method. DNA frament node 59 was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicilin resistance gene..
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:57, 12 October 2023


node59-Y6A

ASR1(node59) is constructed on pET-22b(+) vector by Gibson Assembly method. DNA frament node 59 was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicilin resistance gene..

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]