Difference between revisions of "Part:BBa K4694002"

Line 3: Line 3:
 
<partinfo>BBa_K4694002 short</partinfo>
 
<partinfo>BBa_K4694002 short</partinfo>
 
===Usage and Biology===
 
===Usage and Biology===
The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as Candida albicans and Staphylococcus epidermidis (Weiland-Brauer et al., 2019). The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.  
+
The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as <i>Candida albicans</i> and <i>Staphylococcus epidermidis</i> (Weiland-Brauer et al., 2019). The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.  
  
 
This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT.
 
This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT.
 +
 +
===Characterisation===
 +
 +
 +
===References===
 +
Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of <i>Candida albicans</i> and <i>Staphylococcus epidermidis</i>. <i>PLoS One</i>, 14, e0211366.
  
 
<!-- -->
 
<!-- -->

Revision as of 10:55, 12 October 2023


His-QQ-7

Usage and Biology

The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as Candida albicans and Staphylococcus epidermidis (Weiland-Brauer et al., 2019). The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.

This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT.

Characterisation

References

Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of Candida albicans and Staphylococcus epidermidis. PLoS One, 14, e0211366.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]