Difference between revisions of "Part:BBa K243017"
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This part is the counter part to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010],it inhabits the active cutting site of our universal endonuclease. | This part is the counter part to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010],it inhabits the active cutting site of our universal endonuclease. | ||
We prefer this part because it has an other combiantion of basic parts than His-FluA-Split-Fok_i, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] | We prefer this part because it has an other combiantion of basic parts than His-FluA-Split-Fok_i, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] | ||
| − | The purification is made with a Streptavidin column. | + | The purification is made with a Streptavidin column and the linkage is done by dioxigenin binding. |
Revision as of 18:55, 20 October 2009
Strep-DigA-Split Linker-Fok_a
This part is the counter part to BBa_K243010,it inhabits the active cutting site of our universal endonuclease. We prefer this part because it has an other combiantion of basic parts than His-FluA-Split-Fok_i, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] The purification is made with a Streptavidin column and the linkage is done by dioxigenin binding.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1132