Difference between revisions of "Part:BBa K4837000:Design"

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===Design Notes===
 
===Design Notes===
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It is needed to be used with another biobrick to perform its function.
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<a href='https://static.igem.wiki/teams/4837/wiki/engineering-plasmid.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/engineering-plasmid.png" width="100%"/>
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The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.
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pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.
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Other biobricks such as BBa_K4837001 are required to perform its function. The details of these two basic parts are listed below:
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<a href='https://static.igem.wiki/teams/4837/wiki/part-table.jpg' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/part-table.jpg" width="100%"/>
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===Source===
 
===Source===

Latest revision as of 10:50, 12 October 2023


Lignin peroxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NheI site found at 1694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2352


Design Notes

The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.

pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.

Other biobricks such as BBa_K4837001 are required to perform its function. The details of these two basic parts are listed below:


Source

It is designed based on part of Phanerochaete chrysosporium.

References