Difference between revisions of "Part:BBa K4790103"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4790103 short</partinfo>
 
<partinfo>BBa_K4790103 short</partinfo>
  
−
The DNA fragment has been site-directed mutated through PCR. The 79th amino acid in expression product of node 59 was mutated from glutamine to asparagine.
+
ASR1(node 59)-Q79N is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of ASR1(node 59)-Q79N was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.
  
  

Latest revision as of 10:46, 12 October 2023


ASR1(node 59)-Q79N/pET-22b(+)

ASR1(node 59)-Q79N is constructed on pET-22b(+) vector by Gibson Assembly method. DNA fragment of ASR1(node 59)-Q79N was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is the ampicillin resistance gene.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 395
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 395
    Illegal EcoRI site found at 960
    Illegal NheI site found at 882
    Illegal NheI site found at 1113
    Illegal PstI site found at 261
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 395
    Illegal EcoRI site found at 960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 395
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 395
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]