Difference between revisions of "Part:BBa K4814004"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4814004 short</partinfo> | ||
+ | To further design a system that can detect DNA breaks in vivo, we proposed a FRET (Förster resonance energy transfer) system using the interaction between ATRIP and RPA1. | ||
+ | The ATR (ATMand Rad3-related) and ATRIP (ATR-Interacting protein) form a complex under the circumstances of DNA damage (Qinhui Rao et. al., 2017) (Nam, E. A., & Cortez, D., 2011). | ||
+ | Due to the double-strand breaks (DSBs), replication protein A (RPA) will bind to the single-stranded DNA (ssDNA) and recruit ATRIP complex. An earlier research (Zou, L., & Elledge, S. J., 2003) demonstrated that the RPA-ssDNA-ATRIP interaction is important in checkpoint signaling. To be specific, the RPA70 (RPA1) N-terminus interacts with the ATRIP N-termius (Ball, H. L., Myers, J. S., & Cortez, D., 2005). | ||
+ | |||
+ | * Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814006">ATRIP-EGFP BBa_K4814006</a></html> and <html><a href="https://parts.igem.org/Part:BBa_K4814008">ATRIP-ECFP BBa_K4814008</a></html> | ||
+ | |||
+ | References: | ||
+ | |||
+ | Qinhui Rao et. al. (2017). Cryo-EM structure of human ATR-ATRIP complex. Cell Research (2017) :1-1. | ||
+ | |||
+ | Nam, E. A., & Cortez, D. (2011). ATR signalling: more than meeting at the fork. The Biochemical journal, 436(3), 527–536. https://doi.org/10.1042/BJ20102162 | ||
+ | |||
+ | Ball, H. L., Myers, J. S., & Cortez, D. (2005). ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Molecular biology of the cell, 16(5), 2372–2381. https://doi.org/10.1091/mbc.e04-11-1006 | ||
+ | |||
+ | Zou, L., & Elledge, S. J. (2003). Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science (New York, N.Y.), 300(5625), 1542–1548. https://doi.org/10.1126/science.1083430 | ||
+ | |||
+ | |||
+ | Sequence derived from: https://www.uniprot.org/uniprotkb/Q8WXE1/entry | ||
+ | https://www.ncbi.nlm.nih.gov/gene/84126 | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4814004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4814004 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 10:17, 12 October 2023
ATRIP (human)
To further design a system that can detect DNA breaks in vivo, we proposed a FRET (Förster resonance energy transfer) system using the interaction between ATRIP and RPA1. The ATR (ATMand Rad3-related) and ATRIP (ATR-Interacting protein) form a complex under the circumstances of DNA damage (Qinhui Rao et. al., 2017) (Nam, E. A., & Cortez, D., 2011). Due to the double-strand breaks (DSBs), replication protein A (RPA) will bind to the single-stranded DNA (ssDNA) and recruit ATRIP complex. An earlier research (Zou, L., & Elledge, S. J., 2003) demonstrated that the RPA-ssDNA-ATRIP interaction is important in checkpoint signaling. To be specific, the RPA70 (RPA1) N-terminus interacts with the ATRIP N-termius (Ball, H. L., Myers, J. S., & Cortez, D., 2005).
- Click here for the experiment data: ATRIP-EGFP BBa_K4814006 and ATRIP-ECFP BBa_K4814008
References:
Qinhui Rao et. al. (2017). Cryo-EM structure of human ATR-ATRIP complex. Cell Research (2017) :1-1.
Nam, E. A., & Cortez, D. (2011). ATR signalling: more than meeting at the fork. The Biochemical journal, 436(3), 527–536. https://doi.org/10.1042/BJ20102162
Ball, H. L., Myers, J. S., & Cortez, D. (2005). ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Molecular biology of the cell, 16(5), 2372–2381. https://doi.org/10.1091/mbc.e04-11-1006
Zou, L., & Elledge, S. J. (2003). Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science (New York, N.Y.), 300(5625), 1542–1548. https://doi.org/10.1126/science.1083430
Sequence derived from: https://www.uniprot.org/uniprotkb/Q8WXE1/entry
https://www.ncbi.nlm.nih.gov/gene/84126
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1193
Illegal BsaI.rc site found at 1381
Illegal SapI site found at 2205