Difference between revisions of "Part:BBa K4691002"

 
 
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We obtained a series of RBS sequences Community RBS Collection in the iGEM library, which are suitable for general protein expression in E.coli or other prokaryotes ( including hrpRS protein used in DNA damage detection system ), and cover a series of translation initiation rates. We connected the above sequences with the protein coding sequences of HrpR and HrpS, and predicted the corresponding translation rate through the RBS Calculator, so as to screen out the RBS sequences of HrpR and HrpS proteins that are most suitable for our application for experiments.
 
We obtained a series of RBS sequences Community RBS Collection in the iGEM library, which are suitable for general protein expression in E.coli or other prokaryotes ( including hrpRS protein used in DNA damage detection system ), and cover a series of translation initiation rates. We connected the above sequences with the protein coding sequences of HrpR and HrpS, and predicted the corresponding translation rate through the RBS Calculator, so as to screen out the RBS sequences of HrpR and HrpS proteins that are most suitable for our application for experiments.
  
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The modified RBS30's sequence is CTAGAGATTAAAGAGGAGAATACTAG,and its translation rate we predicted is 2897.89 (au).
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The modified RBS35's sequence is CTAGAGATTAAAGAGGAGAATACTAG,and its translation rate we predicted is 2897.89 (au).
  
  

Latest revision as of 10:17, 12 October 2023


Modified RBS35

We obtained a series of RBS sequences Community RBS Collection in the iGEM library, which are suitable for general protein expression in E.coli or other prokaryotes ( including hrpRS protein used in DNA damage detection system ), and cover a series of translation initiation rates. We connected the above sequences with the protein coding sequences of HrpR and HrpS, and predicted the corresponding translation rate through the RBS Calculator, so as to screen out the RBS sequences of HrpR and HrpS proteins that are most suitable for our application for experiments.

The modified RBS35's sequence is CTAGAGATTAAAGAGGAGAATACTAG,and its translation rate we predicted is 2897.89 (au).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]