Difference between revisions of "Part:BBa K4814013"

 
 
(4 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4814013 short</partinfo>
 
<partinfo>BBa_K4814013 short</partinfo>
  
−
To optimize the design of the RecA-eGFP reporter (BBa_K3020000), we replaced the original B0034 RBS with a new RBS designed by Zhang, M. et al (2022), and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814012 does not enhance the level of fluorescence of the reporter.
+
To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the original B0034 RBS with a new RBS designed by Zhang, M. et al (2022), and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814012 (Strong) does not enhance the level of fluorescence of the reporter.
 +
 
 +
<html>
 +
<table>
 +
<tr>
 +
<td><img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-uvb.png alt=""style="width:400px;height:auto;">
 +
</td>
 +
<td>
 +
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-h2o2.png alt=""style="width:400px;height:auto;">
 +
</td>
 +
</tr>
 +
</table>
 +
<p>RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
 +
<table>
 +
<tr>
 +
<td>
 +
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-na-1.png alt=""style="width:400px;height:auto;">
 +
</td>
 +
<td>
 +
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-ap-1.png alt=""style="width:400px;height:auto;">
 +
</td>
 +
</tr>
 +
</table>
 +
<p>RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
 +
</html>
 +
 
  
 
Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015
 
Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Latest revision as of 09:55, 12 October 2023


RecA(K3)-Strong-eGFP

To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the original B0034 RBS with a new RBS designed by Zhang, M. et al (2022), and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814012 (Strong) does not enhance the level of fluorescence of the reporter.

RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.

RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.


Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]