Difference between revisions of "Part:BBa K4814012"
Line 6: Line 6:
 
Sequence: TTTAAGAGGGGGCTATACAT
 
Sequence: TTTAAGAGGGGGCTATACAT
  
−
<html>
+
Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814013">RecA(K3)-Strong-eGFP BBa_K4814013</a></html>
−
<table>
+
−
<tr>
+
−
<td>
+
−
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-uvb.png alt=""style="width:400px;height:auto;">
+
−
</td>
+
−
<td>
+
−
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-h2o2.png alt=""style="width:400px;height:auto;">
+
−
</td>
+
−
</tr>
+
−
</table>
+
−
<p>RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
+
−
<table>
+
−
<tr>
+
−
<td>
+
−
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-na.png alt=""style="width:400px;height:auto;">
+
−
</td>
+
−
<td>
+
−
<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-ap-1.png alt=""style="width:400px;height:auto;">
+
−
</td>
+
−
</tr>
+
−
</table>
+
−
<p>RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
+
−
</html>
+
  
 
References:
 
References:

Revision as of 09:54, 12 October 2023


Strong RBS

To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. Sequence: TTTAAGAGGGGGCTATACAT

Click here for the experiment data: RecA(K3)-Strong-eGFP BBa_K4814013

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]