Difference between revisions of "Part:BBa K4814010"

Latest revision as of 09:52, 12 October 2023

Mammalian Codon Optimized EYFP

We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.

The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.

Click here for the experiment data: RPA1-EYFP BBa_K4814009

References:

Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0

Sequence derived from: https://www.snapgene.com/plasmids/fluorescent_protein_genes_and_plasmids/EYFP

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
+__NOTOC__
+<partinfo>BBa_K4814010 short</partinfo>
 We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.
 The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.
+Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814009">RPA1-EYFP BBa_K4814009</a></html>
 References: