Difference between revisions of "Part:BBa K4814011"

 
 
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<partinfo>BBa_K4814011 short</partinfo>
 
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We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006.
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Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814007">RPA1-mCherry BBa_K4814007</a></html>
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References
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037
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Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 09:52, 12 October 2023


Mammalian Codon Optimized mCherry

We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006.

Click here for the experiment data: RPA1-mCherry BBa_K4814007

References

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037

Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]