Difference between revisions of "Part:BBa K4814000"

 
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<h1>hha</h1>
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<h1>The hha biofilm reducing protein</h1>
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Hha is a biofilm reducing protein which decreases initial biofilm formation (Hong, S. H., Lee, J., & Wood, T. K., 2010). Hha is a protein of the Hha family, and it is very similar to YmoA protein from Yersinia enterocolitica (Balsalobre, C., et al., 1996). Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., & Juárez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).
<p><span style="color: rgb(0, 0, 0);font-size: 12pt;">Hha is a biofilm reducing protein which decreases initial biofilm formation (</span><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Hong, S. H., Lee, J., &amp; Wood, T. K., 2010)</span><span style="color: rgb(0, 0, 0);font-size: 12pt;">. Hha is a protein of the Hha family, and it is very similar to YmoA protein from&nbsp;</span><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Yersinia enterocolitica (Balsalobre, C., et al., 1996).&nbsp;</span><span style="color: rgb(0, 0, 0);font-size: 12pt;">&nbsp;Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., &amp; Ju&aacute;rez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).</span></p>
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<p><br></p>
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Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814001">BBa_K4814001</a></html>
<p><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Balsalobre, C., Ju&aacute;rez, A., Madrid, C., Mouri&ntilde;o, M., Prenafeta, A., &amp; Mu&ntilde;oa, F. J. (1996). Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage.&nbsp;</span><em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Microbiology (Reading, England)</span></em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">,&nbsp;</span><em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">142 ( Pt 7)</span></em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">, 1841&ndash;1846.&nbsp;</span><a href="https://doi.org/10.1099/13500872-142-7-1841"><u><span style="color: rgb(17, 85, 204);background-color: rgb(255, 255, 255);font-size: 12pt;">https://doi.org/10.1099/13500872-142-7-1841</span></u></a></p>
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<p><br></p>
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Sequence derived from: https://www.uniprot.org/uniprotkb/P0ACE6/entry
<p><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Hong, S. H., Lee, J., &amp; Wood, T. K. (2010). Engineering global regulator Hha of Escherichia coli to control biofilm dispersal.&nbsp;</span><em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">Microbial biotechnology</span></em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">,&nbsp;</span><em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">3</span></em><span style="color: rgb(33, 33, 33);background-color: rgb(255, 255, 255);font-size: 12pt;">(6), 717&ndash;728.&nbsp;</span><a href="https://doi.org/10.1111/j.1751-7915.2010.00220.x"><u><span style="color: rgb(17, 85, 204);background-color: rgb(255, 255, 255);font-size: 12pt;">https://doi.org/10.1111/j.1751-7915.2010.00220.x</span></u></a></p>
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<p><br></p>
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<p><span style="color: rgb(0, 0, 0);font-size: 12pt;">Madrid, C., Nieto, J. M., &amp; Ju&aacute;rez, A. (2001). Role of the Hha/YmoA family of proteins in the thermoregulation of the expression of virulence factors. International Journal of Medical Microbiology, 291(6-7), 425-432.&nbsp;</span><a href="https://doi.org/10.1078/1438-4221-00149"><u><span style="color: rgb(17, 85, 204);font-size: 12pt;">https://doi.org/10.1078/1438-4221-00149</span></u></a></p>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4814000 SequenceAndFeatures</partinfo>
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<p><span style="color: rgb(0, 0, 0);font-size: 12pt;">Sequence derived from: https://www.uniprot.org/uniprotkb/P0ACE6/entry</span></p>
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===Functional Parameters===
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<partinfo>BBa_K4814000 parameters</partinfo>
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Latest revision as of 09:34, 12 October 2023

hha

Hha is a biofilm reducing protein which decreases initial biofilm formation (Hong, S. H., Lee, J., & Wood, T. K., 2010). Hha is a protein of the Hha family, and it is very similar to YmoA protein from Yersinia enterocolitica (Balsalobre, C., et al., 1996). Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., & Juárez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).

Click here for the experiment data: BBa_K4814001

Sequence derived from: https://www.uniprot.org/uniprotkb/P0ACE6/entry

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]