Difference between revisions of "Part:BBa K4759209"

 
 
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Glutathione sulfhydryl transferase (GST), originally discovered in S. japonicum, protein size 26 kDa, is a commonly used soluble affinity purification tag.
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We add a solubilization-promoting tag at the N-teminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
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https://static.igem.wiki/teams/4759/wiki/protein-tags.png
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Fig1: The application of protein tags
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:43, 12 October 2023


T7-RBS4-GST-linker-Olep

Glutathione sulfhydryl transferase (GST), originally discovered in S. japonicum, protein size 26 kDa, is a commonly used soluble affinity purification tag.

We add a solubilization-promoting tag at the N-teminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

protein-tags.png

Fig1: The application of protein tags


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 890
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 177