Difference between revisions of "Part:BBa K4759208"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4759208 short</partinfo>
 
<partinfo>BBa_K4759208 short</partinfo>
  
−
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
+
NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.<br>
 +
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
 +
 
 +
https://static.igem.wiki/teams/4759/wiki/protein-tags.png
 +
 
 +
Fig1: The application of protein tags
 +
 
 +
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 08:41, 12 October 2023


T7-RBS4-Nus-linker-Olep

NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

protein-tags.png

Fig1: The application of protein tags


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1623
    Illegal AgeI site found at 1721
  • 1000
    COMPATIBLE WITH RFC[1000]