Difference between revisions of "Part:BBa K4895201:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This design mainly centers on the correct orientation of BbsI cut sites as well as homologous cutsites to the aformentioned parts in the description.
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This design mainly centers on the correct orientation of BbsI cut sites as well as homologous cutsites to the aforementioned parts in the description.
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The BbsI cut sites are set up such that the recognition sites cut themselves off of the insert. What this means is that, when digested with BbsI, the BbsI sites will be recognized, but then the recognition cuts itself off of the insert. Therefore, after digestion, the insert will no longer contain any BbsI sites.
 
===Source===
 
===Source===
  

Latest revision as of 08:36, 12 October 2023


Insert template for cloning into parts BBa_K4895202, BBa_K4895203


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design mainly centers on the correct orientation of BbsI cut sites as well as homologous cutsites to the aforementioned parts in the description.

The BbsI cut sites are set up such that the recognition sites cut themselves off of the insert. What this means is that, when digested with BbsI, the BbsI sites will be recognized, but then the recognition cuts itself off of the insert. Therefore, after digestion, the insert will no longer contain any BbsI sites.

Source

This part contains an sfGFP ribosome binding site. Otherwise, this part exists as purely synthetic DNA

References