Difference between revisions of "Part:BBa K4837001"

Revision as of 08:33, 12 October 2023

Manganese peroxidase

This plasmid optimized the sequence from white-rot fungus (Phanerochaete chrysosporium) for E. coli expression.

The product illustrated below, namely manganese peroxidase (MnP), works together with a DNA duplex and another product from BBa_K4837000 to form a "cascade enzyme system" for PFAs degradation.

MnP is conjugated with an azide-modified DNA oligo, which is illustrated below:

Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 126
Illegal NotI site found at 781
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 66
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1149
Illegal NgoMIV site found at 1153
Illegal AgeI site found at 651
Illegal AgeI site found at 1132
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 865
Illegal SapI.rc site found at 523

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 <a href='https://static.igem.wiki/teams/4837/wiki/parts-mnp.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/parts-mnp.png" width="30%"/>
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 MnP is conjugated with an azide-modified DNA oligo, which is illustrated below:
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 <a href='https://static.igem.wiki/teams/4837/wiki/description-photo-4.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-4.pngg" width="30%"/>
 </a>
+</html>
 <!-- Add more about the biology of this part here