Difference between revisions of "Part:BBa K4825017:Design"

Revision as of 08:26, 12 October 2023

ATF1

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 412
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 412
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 412
Illegal BglII site found at 99
Illegal BamHI site found at 1426
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 412
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 412
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534

Design Notes

1. A strong promoter pTDH3 was used to maximize the conversion from isopentanol to isoamyl acetate.

Source

Saccharomyces cerevisiae

References

Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970.

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	===References===		===References===
		+	Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970.