Difference between revisions of "Part:BBa K4825004:Design"

Revision as of 08:09, 12 October 2023

Cry1518-35

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 132
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

"1. Codons are optimized for E.coli based when it is expressed in E.coli, and optimized for S.cerevisiae based when it is expressed in S.cerevisiae. 2. 6x His tag (HHHHHH) is added to either the N-terminal or C-terminal of the coding sequence for Ni-NTA purification."

Source

Bacillus thuringiensis

References

孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15.

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	===References===		===References===
		+	孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15.