Difference between revisions of "Part:BBa K4604022"

Latest revision as of 07:57, 12 October 2023

piG_10b (tetR_bluB_T7RBS_riboK12_mazF)

Plasmid piG_10 is made up of the tet promoter/repressor, bluB, a riboswitch for AdoCbl, the tet RBS, mazF (toxin) and the rrnB terminator. The backbone we utilized was pGGAselect, a plasmid commonly used with golden gate cloning that has a chloramphenicol resistance. This BioBrick is BBa_K4604021 with an additional RBS.

Notice

We have performed western blot after induction of this BioBrick, however we could not detect any BluB expression. The reason for this is not known to us, however for the sake of completion and to allow further exploration, we wanted to upload this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 710
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1603
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2252

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 __NOTOC__
 <partinfo>BBa_K4604022 short</partinfo>
 <html>
 Plasmid piG_10 is made up of the tet promoter/repressor, bluB, a riboswitch for AdoCbl, the tet RBS, mazF (toxin) and the rrnB terminator. The backbone we utilized was pGGAselect, a plasmid commonly used with golden gate cloning that has a chloramphenicol resistance. This BioBrick is <a href="https://parts.igem.org/Part:BBa_K4604021">BBa_K4604021</a> with an additional RBS.</html>