Difference between revisions of "Part:BBa K4785004:Design"

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Revision as of 07:51, 12 October 2023


pslg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of PslG was truncated to remove the signal peptide that was originally used to mediate the secretion of PslG in P. aeruginosa, so that PSLG could be better expressed in engineered bacteria.


Source

This part is from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)v

References

Yu, S., Su, T., Wu, H. et al. PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix. Cell Res 25, 1352–1367 (2015). https://doi.org/10.1038/cr.2015.129