Difference between revisions of "Part:BBa K4759209"
JianghuaChen (Talk | contribs) |
|||
Line 3: | Line 3: | ||
<partinfo>BBa_K4759209 short</partinfo> | <partinfo>BBa_K4759209 short</partinfo> | ||
− | + | we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. | |
− | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 07:40, 12 October 2023
T7-RBS4-GST-linker-Olep
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 890 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 177