Difference between revisions of "Part:BBa K4893001"
 
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A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using  E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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PQQGDH is a dimeric enzyme produced in E. coli. It is responsible for oxidizing glucose into glono-d-lactone which is then hydrolyzed into gluconic acid. In its halo form, it possesses the PQQ cofactor that gives its redox abilities.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4893001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4893001 SequenceAndFeatures</partinfo>
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SDS page of Crude Sample of PQQGDH
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https://static.igem.wiki/teams/4893/wiki/bba-k4893001-sds-page.jpg
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Modeling of PQQGDH in PyMOL
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https://static.igem.wiki/teams/4893/wiki/bba-k4893001-model.jpg
  
  

Latest revision as of 05:50, 12 October 2023


Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

SDS page of Crude Sample of PQQGDH bba-k4893001-sds-page.jpg

Modeling of PQQGDH in PyMOL bba-k4893001-model.jpg