Difference between revisions of "Part:BBa K4893001"

Latest revision as of 05:50, 12 October 2023

Pyrroloquinoline Quinone Glucose Dehydrogenase

A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 450
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

SDS page of Crude Sample of PQQGDH

Modeling of PQQGDH in PyMOL

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4893001 short</partinfo>
-test
+A.calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using  E. coli. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives the protein its electron-carrying abilities. This enzyme has been most widely used for glucose-sensing applications. In our project, we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-testing 123 123
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4893001 SequenceAndFeatures</partinfo>
+SDS page of Crude Sample of PQQGDH
+https://static.igem.wiki/teams/4893/wiki/bba-k4893001-sds-page.jpg
+Modeling of PQQGDH in PyMOL
+https://static.igem.wiki/teams/4893/wiki/bba-k4893001-model.jpg