Difference between revisions of "Part:BBa K4893001:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to | + | We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubate PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor. |
===Applications of BBa_K4893001=== | ===Applications of BBa_K4893001=== | ||
Latest revision as of 05:44, 12 October 2023
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how you used this part and how it worked out.
We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubate PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor.
Applications of BBa_K4893001
Glucose sensors, enzyme fuel cells
User Reviews
UNIQ1d3fe0f7ac190bb7-partinfo-00000000-QINU UNIQ1d3fe0f7ac190bb7-partinfo-00000001-QINU