Difference between revisions of "Part:BBa K4585010"
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<partinfo>BBa_K4585010 short</partinfo> | <partinfo>BBa_K4585010 short</partinfo> | ||
| − | The purpose of this linear vector is for subsequent homologous recombination synthesis of the pGL4.35- | + | The purpose of this linear vector is for subsequent homologous recombination synthesis of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid. |
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| + | <h2 class="pageContent-main__title"> | ||
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| + | pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector | ||
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| + | <p>We based on the sequence of the pGL4.35-3×HA-9×GAL4UAS-KRAB-NLS plasmid. Special primers were designed to cut the plasmid into a linear sequence of length of about 5338 bp by PCR. The two ends of this linear sequence can be complementary to the Degron homologous recombination fragment of the homologous Degron fragment for homologous recombination to obtain the target product pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid. | ||
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| + | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
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| + | 1 Diagrams | ||
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| + | <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/pgl4-35-3-ha-9-gal4uas-krab-degron-nls-linearized-vector/pgl4-35-3-ha-9-gal4uas-krab-degron-nls-linearized-vector.png"></p> | ||
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| + | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The agarose gel electrophoresis of pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector</p> | ||
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| + | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
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| + | 2 Caution | ||
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| + | <p>The two ends of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vectror need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4585010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4585010 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 03:58, 12 October 2023
pGL4.35-3xHA-9xGAL4UAS-KRAB-Degron-NLS linearized vector
The purpose of this linear vector is for subsequent homologous recombination synthesis of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid.
pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector
We based on the sequence of the pGL4.35-3×HA-9×GAL4UAS-KRAB-NLS plasmid. Special primers were designed to cut the plasmid into a linear sequence of length of about 5338 bp by PCR. The two ends of this linear sequence can be complementary to the Degron homologous recombination fragment of the homologous Degron fragment for homologous recombination to obtain the target product pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid.
1 Diagrams
Fig.1 The agarose gel electrophoresis of pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector
2 Caution
The two ends of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vectror need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4433
Illegal XbaI site found at 161
Illegal XbaI site found at 1943
Illegal XbaI site found at 4270
Illegal XbaI site found at 4505
Illegal SpeI site found at 4029
Illegal PstI site found at 3118 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4433
Illegal NheI site found at 4461
Illegal SpeI site found at 4029
Illegal PstI site found at 3118
Illegal NotI site found at 3097 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4433
Illegal BglII site found at 4508
Illegal BamHI site found at 423
Illegal BamHI site found at 5100
Illegal BamHI site found at 5325
Illegal XhoI site found at 4313
Illegal XhoI site found at 4870 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4433
Illegal XbaI site found at 161
Illegal XbaI site found at 1943
Illegal XbaI site found at 4270
Illegal XbaI site found at 4505
Illegal SpeI site found at 4029
Illegal PstI site found at 3118 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4433
Illegal XbaI site found at 161
Illegal XbaI site found at 1943
Illegal XbaI site found at 4270
Illegal XbaI site found at 4505
Illegal SpeI site found at 4029
Illegal PstI site found at 3118
Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 176
Illegal NgoMIV site found at 1654
Illegal NgoMIV site found at 1671
Illegal NgoMIV site found at 1803
Illegal NgoMIV site found at 1894
Illegal NgoMIV site found at 2146
Illegal AgeI site found at 1949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4789
Illegal SapI site found at 444
Illegal SapI site found at 2155
Illegal SapI.rc site found at 5283