Difference between revisions of "Part:BBa K4683008"
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<partinfo>BBa_K4683008 short</partinfo> | <partinfo>BBa_K4683008 short</partinfo> | ||
− | This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b is used as the basis | + | This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b (<partinfo>BBa_K4683005 </partinfo>)is used as the basis for the 3' arm (<partinfo>BBa_K4683006 </partinfo>) and 5' arm (<partinfo>BBa_K4683007 </partinfo>), |
− | When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations. | + | When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from <partinfo>BBa_K4245131 </partinfo> (Middle Sequence), which is then detected by the linear probes <partinfo>BBa_K4245130 </partinfo> (Fluorophore) and <partinfo>BBa_K4245132 </partinfo> (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations. |
Latest revision as of 03:43, 12 October 2023
hsa-miR-20b Padlock Probe
This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b (BBa_K4683005)is used as the basis for the 3' arm (BBa_K4683006) and 5' arm (BBa_K4683007), When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]