Difference between revisions of "Part:BBa K4960005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins.[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)<br> | + | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins.[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure 1''')<br> |
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| − | Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> | + | '''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> |
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The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process. | The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process. | ||
Revision as of 03:31, 12 October 2023
pvc5
guide the synthesis of the PVC tube initiator
Profile
Name: pvc5
Base Pairs:459 bp
Origin:Photorhabdus
Properties: guide the synthesis of the PVC tube initiator
Usage and Biology
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins.[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)
The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.