Difference between revisions of "Part:BBa K4619001"
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<partinfo>BBa_K4619001 short</partinfo>
 
<partinfo>BBa_K4619001 short</partinfo>
  
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PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator [https://parts.igem.org/Part:BBa_K4271007BBa_K4271007]on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter [https://parts.igem.org/Part:BBa_K4271005BBa_K4271005].One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
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PobR is a protein that functions as a transcriptional activator. It first attaches to the PobR operator(<a href="https://parts.igem.org/Part:BBa_K4271007">BBa_K4271007</a>)on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the PobA side of the dual-directional PobA/R promoter(<a href="https://parts.igem.org/Part:BBa_K4271005">BBa_K4271005</a>). One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
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===Usage and Biology===
 
  
 
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Latest revision as of 03:16, 12 October 2023


PobR

PobR is a protein that functions as a transcriptional activator. It first attaches to the PobR operator(BBa_K4271007)on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the PobA side of the dual-directional PobA/R promoter(BBa_K4271005). One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 462
    Illegal AgeI site found at 154
    Illegal AgeI site found at 675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 595