Difference between revisions of "Part:BBa K4619001"
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| − | PobR is a protein that functions as a transcriptional activator. It first attaches to the | + | PobR is a protein that functions as a transcriptional activator. It first attaches to the PobR operator(<a href="https://parts.igem.org/Part:BBa_K4271007">BBa_K4271007</a>)on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the PobA side of the dual-directional PobA/R promoter(<a href="https://parts.igem.org/Part:BBa_K4271005">BBa_K4271005</a>). One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:15, 12 October 2023
PobR
PobR is a protein that functions as a transcriptional activator. It first attaches to the PobR operator(BBa_K4271007)on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the PobA side of the dual-directional PobA/R promoter(BBa_K4271005). One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 462
Illegal AgeI site found at 154
Illegal AgeI site found at 675 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 595