Difference between revisions of "Part:BBa K4815011:Experience"
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===Applications of BBa_K4815011=== | ===Applications of BBa_K4815011=== | ||
| + | We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:07, 12 October 2023
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Applications of BBa_K4815011
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast.
User Reviews
UNIQ00255a597422b115-partinfo-00000000-QINU UNIQ00255a597422b115-partinfo-00000001-QINU