Difference between revisions of "Part:BBa K4591009"
Line 5: Line 5:
 
===Usage and Biology===
 
===Usage and Biology===
 
<html>
 
<html>
−
<p>The part is mainly used to detect and report the presence of PA and TPA, and visually monitor the expression through the GFP and RFP.During the strain is put into use, we can regulate the flipping sequence to detect the presence of TPA and activate the expression of GFP.</p>
+
<p>In order to observe the decomposition of PET by engineered bacteria, our team designed this plasmid. The plasmid mainly consists of RFP, lox66&lox71, PHpaII, Xylsmut, tetO and sfGFP genes. It is mainly used to detect and report the presence of PA and TPA, and visually monitor the expression through the GFP.When the TPA is detected by the Xylsmut, the downstream genes will be activated and transcribed——like the strain will express green fluorescence. </p>
−
<p>In other situations, flipping expresses the RFP and plays an alternative role.Considering that in future product applications, the strain may not necessarily be used to degrade PET immediately after delivery, we designed the flipping system to cope with different use scenarios. For example, when applied in a factory, in order to reduce adverse effects during fermentation production, the engineered bacteria express antibacterial products before turning over, and then turn over to express downstream degradation modules until they are put into use. Or in the field can choose to flip the expression before the production of soil fertility of the substance.
+
<p>What’s more, considering that in future product applications, the strain may not necessarily be used to degrade PET immediately after delivery, we designed the flipping system to cope with different use situations. During the strain is put into use to decompose the PET, we can regulate the flipping sequence to detect the presence of TPA and activate the expression of GFP. In other situations, flipping expresses the RFP and plays an alternative role. For example, when applied in a factory, in order to reduce adverse effects during fermentation production, the engineered bacteria express antibacterial products before turning over, and then turn over to express downstream degradation modules until they are put into use. Or in the field, the strain produces substances that increase fertility into the soil before the sequence is flipped. In turn, the engineered bacteria could have more purposes.
−
<p> In turn, the engineered bacteria can have more uses.</p>
+
</p>
  
−
 
−
 
−
 
−
</p>
 
 
</html>
 
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:31, 12 October 2023


T500-RFP-lox66-Hpall-lox71-XylSmut-tetO-sfGFP-T500

Usage and Biology

In order to observe the decomposition of PET by engineered bacteria, our team designed this plasmid. The plasmid mainly consists of RFP, lox66&lox71, PHpaII, Xylsmut, tetO and sfGFP genes. It is mainly used to detect and report the presence of PA and TPA, and visually monitor the expression through the GFP.When the TPA is detected by the Xylsmut, the downstream genes will be activated and transcribed——like the strain will express green fluorescence.

What’s more, considering that in future product applications, the strain may not necessarily be used to degrade PET immediately after delivery, we designed the flipping system to cope with different use situations. During the strain is put into use to decompose the PET, we can regulate the flipping sequence to detect the presence of TPA and activate the expression of GFP. In other situations, flipping expresses the RFP and plays an alternative role. For example, when applied in a factory, in order to reduce adverse effects during fermentation production, the engineered bacteria express antibacterial products before turning over, and then turn over to express downstream degradation modules until they are put into use. Or in the field, the strain produces substances that increase fertility into the soil before the sequence is flipped. In turn, the engineered bacteria could have more purposes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2580
    Illegal XhoI site found at 2261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2580
    Illegal PstI site found at 2574
    Illegal NgoMIV site found at 2255
    Illegal AgeI site found at 73
    Illegal AgeI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]