Difference between revisions of "Part:BBa K4830018:Design"
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===Design Notes===
 
===Design Notes===
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The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and backbone into plasmid.
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No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
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===Source===
 
===Source===
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===References===
 
===References===
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Pr160 [mouse mammary tumor virus] - protein - NCBI (no date) National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/AAC82556.1?report=genbank&log%24=protalign&blast_rank=1&RID=JD4PC8M2013 (Accessed: 12 October 2023).

Latest revision as of 02:16, 12 October 2023


MMTV RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: AAC82556

References

Pr160 [mouse mammary tumor virus] - protein - NCBI (no date) National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/AAC82556.1?report=genbank&log%24=protalign&blast_rank=1&RID=JD4PC8M2013 (Accessed: 12 October 2023).