Difference between revisions of "Part:BBa K216010:Experience"

Latest revision as of 14:14, 20 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K216010

This part was created to test promoter BBa_R0082 using Miller assays, as a backup to the GFP-based promoter characterization kit.

User Reviews

Initial results: Edinburgh iGEM 2009: To test this part (and thus promoter BBa_R0082), E. coli JM109/pSB1A2-BBa_K216010 was grown overnight in LB with ampicillin (80 mg/l) at 37 C with shaking, in the presence of different concentrations of procaine (which activates EnvZ, the sensor kinase which phosphorylates OmpR, the response regulator which activates this promoter). Useful results were not obtained, possibly due to the very high background activity of the promoter in the absence of added procaine. Better results were obtained using the GFP-based promoter characterization kit: see the BBa_R0082 page for details of these results.

UNIQ43c08c2bdb4b7611-partinfo-00000000-QINU UNIQ43c08c2bdb4b7611-partinfo-00000001-QINU

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 ===User Reviews===
+'''Initial results: Edinburgh iGEM 2009:''' To test this part (and thus promoter BBa_R0082), ''E. coli'' JM109/pSB1A2-BBa_K216010 was grown overnight in LB with ampicillin (80 mg/l) at 37 C with shaking, in the presence of different concentrations of procaine (which activates EnvZ, the sensor kinase which phosphorylates OmpR, the response regulator which activates this promoter). Useful results were not obtained, possibly due to the very high background activity of the promoter in the absence of added procaine. Better results were obtained using the [https://parts.igem.org/Measurement GFP-based promoter characterization kit]: see [https://parts.igem.org/Part:BBa_R0082 the BBa_R0082 page] for details of these results.
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