Difference between revisions of "Part:BBa K4830017:Design"

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===Design Notes===
 
===Design Notes===
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The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
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No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654.

Latest revision as of 01:42, 12 October 2023


Ll.ltrA RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: RQG78521

References

López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654.