Difference between revisions of "Part:BBa K4788000"
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This is a promoter that responds to the inhibitory effect of FadR by adding the Fad O operator afterwards. | This is a promoter that responds to the inhibitory effect of FadR by adding the Fad O operator afterwards. | ||
| − | <!-- Add more about the biology of this part here> | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | It is a promoter that can combine with the FadO operator and FadR gene to form an oleic acid inducer.For specific information on oleic acid inducers, please refer to <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4788002"> | + | It is a promoter that can combine with the FadO operator and FadR gene to form an oleic acid inducer.For specific information on oleic acid inducers, please refer to <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4788002"> BBa_K4788002</a ></html> |
===After forming composite components=== | ===After forming composite components=== | ||
The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects. | The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects. | ||
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| + | ===Reference=== | ||
| + | Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297. | ||
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Latest revision as of 01:25, 12 October 2023
FadBA promoter
This is a promoter that responds to the inhibitory effect of FadR by adding the Fad O operator afterwards.
Usage and Biology
It is a promoter that can combine with the FadO operator and FadR gene to form an oleic acid inducer.For specific information on oleic acid inducers, please refer to BBa_K4788002
After forming composite components
The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.
Reference
Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]