Difference between revisions of "Part:BBa K4815021:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | After testing our model by the dual fluorescence, to furtherly prove our learning model’s ability, we engineered the promoter sequence of S. cerevisiae to produce E. coli heat-labile enterotoxin subunit B (LTB). Our goal is using our AI to tackle the issue of the low LTB production yield. By inserting the synthesized efficient high expression or the low expression promoters, it can generate the expression rate of the LTB which can be detected by the GFP. The western blot and the RT-PCR were adopted to test. | |
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===Source=== | ===Source=== | ||
| − | + | Heat-labile enterotoxin subunit B (LTB) which is expressed by E. coli is one of the most popular oral vaccine adjuvants and intestine adsorption enhancers. We fused LTB to eGFP and we tested our expression system for eGFP as a model reporter protein. | |
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Latest revision as of 01:18, 12 October 2023
LTB-eGFP-fusion protein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 298
Design Notes
After testing our model by the dual fluorescence, to furtherly prove our learning model’s ability, we engineered the promoter sequence of S. cerevisiae to produce E. coli heat-labile enterotoxin subunit B (LTB). Our goal is using our AI to tackle the issue of the low LTB production yield. By inserting the synthesized efficient high expression or the low expression promoters, it can generate the expression rate of the LTB which can be detected by the GFP. The western blot and the RT-PCR were adopted to test.
Source
Heat-labile enterotoxin subunit B (LTB) which is expressed by E. coli is one of the most popular oral vaccine adjuvants and intestine adsorption enhancers. We fused LTB to eGFP and we tested our expression system for eGFP as a model reporter protein.