Difference between revisions of "Part:BBa K4645000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | On the basis of the expression of functional target proteins, | + | On the basis of the expression of functional target proteins, His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification. |
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===Source=== | ===Source=== | ||
| − | Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a | + | Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a His tag and a Flag tag at the C-terminus. |
===References=== | ===References=== | ||
Latest revision as of 01:17, 12 October 2023
FelD: Felis domesticus allergen
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 467
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
On the basis of the expression of functional target proteins, His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification.
Source
Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a His tag and a Flag tag at the C-terminus.