Difference between revisions of "Part:BBa K4645000:Design"
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===References===
 
===References===
 +
<p>[1]Kaiser L, Grönlund H, Sandalova T, Ljunggren HG, van Hage-Hamsten M, Achour A, Schneider G. The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family. J Biol Chem. 2003 Sep 26;278(39):37730-5. </p>
 +
<p>[2] Rogers BL, Morgenstern JP, Garman RD, Bond JF, Kuo MC. Recombinant Fel d.I: Expression, purification, IgE binding and reaction with cat-allergic human T cells. Molecular Immunology. 1993 Apr;30(6):559-568. </p>
 +
<p>[3] Kaiser L, Grönlund H, Sandalova T, Ljunggren HG, Schneider G, van Hage-Hamsten M, Achour A. Production, crystallization and preliminary crystallographic study of the major cat allergen Fel d 1. Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1103-5. </p>

Revision as of 01:17, 12 October 2023


FelD: Felis domesticus allergen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag and Flag tag are added, which can help us better obtain high-purity target proteins and do subsequent experimental verification.


Source

Fel d 1 is a protein consisting of two amino acid chains encoded in the genome of domestic feiline. FelD is a recombinant protein formed by fusing 93 amino acids from chain 2 and 70 amino acids from chain 1 of Fel d 1, and with a His tag and a Flag tag at the C-terminus.

References

[1]Kaiser L, Grönlund H, Sandalova T, Ljunggren HG, van Hage-Hamsten M, Achour A, Schneider G. The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family. J Biol Chem. 2003 Sep 26;278(39):37730-5. 

[2] Rogers BL, Morgenstern JP, Garman RD, Bond JF, Kuo MC. Recombinant Fel d.I: Expression, purification, IgE binding and reaction with cat-allergic human T cells. Molecular Immunology. 1993 Apr;30(6):559-568. 

[3] Kaiser L, Grönlund H, Sandalova T, Ljunggren HG, Schneider G, van Hage-Hamsten M, Achour A. Production, crystallization and preliminary crystallographic study of the major cat allergen Fel d 1. Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1103-5.