Difference between revisions of "Part:BBa K4676002:Design"
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1. Lenon, M., Ke, N., Szady, C., Sakhtah, H., Ren, G., Manta, B., Causey, B., & Berkmen, M. (2020, September 30). Improved production of Humira antibody in the genetically engineered escherichia coli shuffle, by co-expression of human PDI-GPX7 fusions - Applied Microbiology and Biotechnology. SpringerLink. https://link.springer.com/article/10.1007/s00253-020-10920-5 | 1. Lenon, M., Ke, N., Szady, C., Sakhtah, H., Ren, G., Manta, B., Causey, B., & Berkmen, M. (2020, September 30). Improved production of Humira antibody in the genetically engineered escherichia coli shuffle, by co-expression of human PDI-GPX7 fusions - Applied Microbiology and Biotechnology. SpringerLink. https://link.springer.com/article/10.1007/s00253-020-10920-5 | ||
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| + | 2. Ibid | ||
Revision as of 00:48, 12 October 2023
Design Notes
The researchers created the protein by "a fusion of two endoplasmic reticulum-resident proteins, the thiol peroxidase GPx7 and the protein disulfide isomerase, PDI." (1)
Source
The sequence for the fusion protein was published as supplementary information by Lénon et al. in their paper "Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions." (2)
References
1. Lenon, M., Ke, N., Szady, C., Sakhtah, H., Ren, G., Manta, B., Causey, B., & Berkmen, M. (2020, September 30). Improved production of Humira antibody in the genetically engineered escherichia coli shuffle, by co-expression of human PDI-GPX7 fusions - Applied Microbiology and Biotechnology. SpringerLink. https://link.springer.com/article/10.1007/s00253-020-10920-5
2. Ibid