Difference between revisions of "Part:BBa K4645002:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins.Additionally, an OmpA signaling peptide was fused to the N terminal of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
+
On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.
+
Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.
  
 
===References===
 
===References===

Latest revision as of 00:30, 12 October 2023


NeuB: scFv ( single-chain fragment variable）that inhibits the immune response to the feline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal XhoI site found at 276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.


Source

Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.

References