Difference between revisions of "Part:BBa K4645001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins.  
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On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
  
  
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===Source===
 
===Source===
  
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Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies was obtained through literature review.  
+
Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN 1908 was obtained through literature review.  
  
 
===References===
 
===References===

Latest revision as of 00:25, 12 October 2023


NeuA: scFv ( single-chain fragment variable）that inhibits the immune response to the feline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 315
    Illegal XhoI site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 315
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.


Source

Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies.The amino acid sequence of one of the most effective monoclonal antibodies REGN 1908 was obtained through literature review.

References